Spatial Mapping of Gene Expression at Single Cell Resolution with the RNAscope Technology

RNAscope, in situ, NIF, Neurobiology Imaging Facility

Comprehensive spatial studies require tools that enable detection of multiple cell types and states. Interrogation of complex tissue types requires a highly sensitive, specific, and multiplexed spatial approach with single cell resolution. In this presentation researchers learn how to use the RNAscope in situ hybridization technology to

  • Visualize cellular heterogeneity and maintain morphology of complex organs with multiplexing and spatial capabilities
  •  Conserve precious samples by interrogating up to 12 targets on a single slide
  • Spatially map scRNA-seq gene profiles at the single cell level in the tissue context
  • Localize newly identified cell subtypes and cell markers

Applications in: cancer, neuroscience, gene therapy, infectious disease, endocrinology, cardiovascular, immune mediated disease, liver, development, CAR-T, stem cell research.

Speaker: Nidhi Vashistha, Ph.D. Senior Scientist, Advanced Cell Diagnostics
October 8, 2019 11AM- 12 PM DFCI, Belfer Center LC4054, 360 Longwood Ave.
October 8, 2019 2 PM- 3 PM HMS Goldenson Bld. Room 122
October 9, 2019 10 AM- 12 PM MGH Charlestown 149 13th St. room 7:139
Guest Speaker: Juan Manuel Gonzalez Rosa, Ph.D. Myocardial Polyploidization Creates a Barrier to Heart Regeneration in Zebrafish

October 9, 2019 3 PM- 4 PM MGH Main Campus Simches Bld. Room 4250
October 10, 2019 2 PM- 3 PM Boston Univ. School of Medicine L Bld. Rm L212
October 11, 2019 10AM- 12 PM MIT McGovern Institute Bld. 46-5165
Guest Speaker: Marie Didiot, Ph.D. Nuclear Localization of Huntingtin mRNA Is Specific to Cells of Neuronal Origin

For more information, contact Jane Clarke
For Research Use Only. Not for diagnostic use. RNAscope® is a registered trademark of Advanced Cell Diagnostics, Inc. in the United States or other countries.
All rights reserved. ©2019 Advanced Cell Diagnostics, Inc.