Microscopy Talks: Detectors, SIM & STED - oh my!

Wed 23/Oct in CLS 13, starting at 10:00a

Talk A - Phil Allen: 10a-11a - Cameras! - "Cameras for imaging in Biosciences"
Talk B - Carlas Smith: 11:00-12:00 - SIM (super-resolution) - "Uniting Structured Illumination Microscopy and Localization Microscopy - SIMFLUX"
Catered Lunch [12:00 -1:00p]
Break [1:00-2:00p]
Talk C - Christine Hanko: 2:00-3:00p (questions ~3:00p-3:10p) - STED (super-resolution) - "HyD and what is means for the perfect STED acquisition"

Abstract -

MINFLUX offers a breakthrough in single molecule localization precision, but suffers from a tiny field of-view and a lack of practical parallelism. SIMFLUX makes use of extended illumination patterns similar to those used in Structured Illumination Microscopy (SIM). Here, we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micron sized field-of-view. We show a near twofold improvement in precision over standard localization microscopy with the same photon count on DNA-origami nano-structures and Tubulin, imaged with PAINT and dSTORM.
Please feel free to reach out to the Cellular Imaging Core (cvic.innocent@childrens.harvard.edu) for questions.
Figure 1 | Principle of SIMFLUX. A total of 6 images are recorded with 3 shifted patterns per orthogonal orientation of the line pattern. Combining the centroid estimates of the 6 frames with the photon count in relation to the pattern shift improves the localization precision with a factor of around two compared to the standard centroid estimate on the sum of the 6 frames.