Array Tomography Core

The Core has recently been awarded a P30 NIH core grant. This grant enables our facility to reduce rates for all users of the AT core. Effective Aug 1, 2017 our pricing for services have been reduced. Please scroll down to see the new adjusted pricing.

The Array Tomography Core is supported by the Neural Imaging Center as part of an NINDS P30 Core Center grant #NS072030.

Please sign in to our database to request AT services

https://ppms.us/hms-neuroimaging/

Please contact Mahmoud el_Rifai to schedule your first validation meeting.

Hours of Operation: Monday – Friday 9am – 5pm

Samples must be dropped off during working hours.

Users will purchase the primary and secondary AB for their image array unless there is a validating collaboration with a vendor. Please inquire.

Offline workstations with Amira and ImageJ are provided for post acquisition.

Long term data storage is not provided. Users must use their own collaboration folders on the HMS server or save their data on a personal device.

Mahmoud el_Rifai
Array Tomography Technician
Goldenson Building – Room 243
617.432.1683
mahmoud_el_rifai@hms.harvard.edu

Array Tomography Protocols

Sample preparation protocol

Please have your sample perfused, fixed and washed according to the protocol prior to drop off. You are responsible for selecting the area to be imaged. You will inform the Array Tomography technician of your intention for ribbon thickness. The AT technician will work with you on the orientation.

1) Your sample will be cut into ribbons at your preferred thickness.

2) Ribbons will be stained with approved antibodies. Antibody list and staining order can be located below. Please note that you are not restricted to the list of antibodies below, however we cannot guarantee that other antibodies will work well with LR white sections.

Approved Primary Antibodies

Staining Sequence

3) All staining will be done by the AT technician in a clean environment to decrease imaging artifacts. Images will be captured and you will receive the raw data along with the final z-stack. Analysis can be done on one of our workstations with Amira software or you maycontact IDAC for more complex analysis.

This facility is supported by the Neural Imaging Center as part of an NINDS P30 Core Center grant #NS072030. All publications must acknowledge this P30 grant number. Please Contact NIF and tell us when you publish your data. We must keep track of all publications as stipulated by our grant.

Service Fees

In accordance with Harvard University Finance Rules: Rate changes will occur July 1, 2017. Harvard requires service centers (cores) to evaluate fee structures and determine fees based on public funding rules and non-profit status. Rates are determined by the cost of maintaining equipment, personnel salary and benefits, square footage and hours used. The facility has been awarded a core grant through NIH. Effective Sept 1, 2017 the NINDS core grant will support the core. NINDS grant holders receive priority and subsidy for their projects.

The pricing is broken down info projects and cost/project External users are required to pay F&A (Facilities and Administrative cost) to Harvard University. This price is set by Harvard and is $99/sqft by the number of total hours external users are using the space.

NINDS = National Institute of Neurological Disorders and Stroke - NIH grant holders

 

Service NINDS Internal (Harvard Univ including HSDM, HSPH and HMS) NINDS External (Hospitals and other universities and institutions) non-NINDS Internal (Harvard Univ including HSDM, HSPH and HMS) non-NINDS External (Hospitals and other universities and institutions)
LR White Embedding $200 $63 for up to 10 blocks
$350 $108 for 10 to 30 blocks
$500 $156 for more than 30 blocks
$400 $130 for up to 10 blocks
$700 $224 for 10 to 30 blocks
$1000 $322 for more than 30 blocks
$200 $107 for up to 10 blocks
$350 $185 for 10 to 30 blocks
$500 $266 for more than 30 blocks
$400 $151 for up to 10 blocks
$700 $262 for 10 to 30 blocks
$1000 $385 for more than 30 blocks
Ultramicrotome Sectioning $75 $73/hour $200 $130/hour $105/hour $185/hour
Staining and Elution $252 $55/slide $550 $401/slide $252 $127/slide $550 $473/slide
Imaging $42 $7/hour $32 $20/hr $42 /hr $83 /hr
Antibody Validation $218 $72/Antibody $550 $340/antibody $218 $124 /Antibody $550 $392/antibody
Image Alignment $1300 $98/project $1300 $1200/project $1300 $488/project $2100 $1658/project

We are more than happy to show you how to image and align your images to save cost. This frees up our technician for the more critical steps of AT (allowing him to take on more projects). Please inquire for training.